Chlorantraniliprole induces adipogenesis in 3T3-L1 adipocytes via the AMPKa pathway but not the ER stress pathway

摘要

Flubendiamide, a ryanoid family insecticide, has been linked to elevated adipogenesis. However, the influence on adipogenesis of chlorantraniliprole, another widely-used ryanoid-class insecticide in agriculture, remains unknown. Therefore, the influence of chlorantraniliprole on adipogenesis was studied in 3T3-L1 cells. Chlorantraniliprole enhanced TG content and upregulated the expression of C/EBP alpha, PPAR gamma, and ACC, three key adipogenic regulators. Chlorantraniliprole decreased the phosphorylation of AMPK, while it had no influence on the expression of endoplasmic reticulum stress regulators. The influence of chlorantraniliprole on adipogenesis was abolished by AMPK alpha activation. Collectively, the results indicate that chlorantraniliprole enhances adipogenesis through the AMPK alpha pathway, but not the ER stress-mediated pathway.