Determination of pyrrolizidine alkaloids in honey from selected sites by solid phase extraction and HPLC-MS

摘要

A method was developed for the determination in honey of the Ragwort (Senecio jacobaea) derived pyrrolizi- dine alkalids jacoline, jacozine, jacobine, seneciphyl- line and senecionine, combining solid-phase extraction with high performance liquid chromatograpohy and atmospheric pressures chemical ionization mass spectro- metric detection. The method allowed determination of individual alkaloids and offered a considerable improve- ment in terms of speed, sensitivity and specificity over previous approaches, but was not suitable for determi- nation of jaconine, a minor alkaloid in Ragwort.