Aflatoxin M_1 occurrence in samples of Grana Padano cheese

摘要

A total of 223 samples of Grana Padano cheese manufactured in 4 years (199-94) by dairies in 11 provinces of the Po valley were checked for aflatoxin M_1. Grated cheese was pextracted with chloroform and The defatted extract was purified by an immunoaffinity Column; aflatoxin M_1 was determined by HPLC using A fluorescence detector. From the analysis of the data it Has emerged that only one sample exceeded the maxi- Has emerged that only one samples exceeded the maxi- Mum tolerated level in cheese in some European Countries (250 ng/kg). Most samples (91/100) were in The range 5-100 ng/kg and only 15 (6.7/100) in the range 100-250 ng/kg.