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Development of an ELISA for maduramicin and determination of the depletion kinetics of maduramicin residues in poultry

机译:maduramicin ELISA的开发以及家禽中maduramicin残留消耗动力学的测定

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Polyclonal antibodies against the coccidiostat madur- amicin were produced by the immunization of rabbits with a conjugate between maduramicin and human erum albumin. The antibodies exhibited minimal cross-reactivity with structurally similar ionophoric coccidiostats (monensin, salinomycin, narasin and laslocid). An enzyme-linked immunosorbent assay (ELISA) was developed for maduramicin in poultry tissue using these antibodies. The ELISA had a limit of detection of approximately 0.02 ng/g in tissue (ng/ml in serum) and a limit of determination of 1.0 ng/g in tissue (ng/ml in serum).
机译:通过用马杜拉米菌素和人血清白蛋白之间的结合物免疫兔,可以产生抗球虫霉素马达霉素的多克隆抗体。抗体与结构相似的离子载体抗球虫药(莫能菌素,沙利霉素,萘灵和拉西洛德)表现出最小的交叉反应性。使用这些抗体开发了一种用于家禽组织中马杜菌素的酶联免疫吸附测定(ELISA)。 ELISA在组织中的检测下限约为0.02 ng / g(在血清中为ng / ml),在组织中的检测下限为1.0 ng / g(在血清中为ng / ml)。

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