Establishment of Specific Pathogen-Free Guinea-pig Colonies Using Limited-Flora Guinea-pigs Associated With Conventional Guinea-pig Flora, and Monitoring of their Cecal Flora

摘要

Six groups of limited flora(LF)Hartley guinea-pigs were produced by inoculation of hysterectomy-derived GF guinea-pigs with various combinations of cecal bacteria of conventional(CV)guinea-pigs to determine the effective bacterial cocktails for the estabishment of a specific pathogen free(SPF)clony. Bifidobacterium magunm(Bif) isolated from CV guinea-pigs was used for pretreatment.