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A Simple Method of Intestinal Anastomosis (Ileocolostomy) in Rats

机译:大鼠肠吻合术(回肠造口术)的简单方法

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A simple method of ileocolostomy was performed in rats. The colon near the cecum was ligated, including its arteries and veins. Main artery and vein of the cecum were ligated. Then, the cecum was cut out. A longitudinal incision was made on the anti-mesenteric side of the proximal end of the colon, approximately 7-8 mm long. A 21-G needle was inserted toward the incision 2 cm away from the proximal end of the anti-mesenteric side of the colon. A nylon suture was knotted once to the distal end of the ileum and was introduced into the tip of the needle which had previously been passed through the colon. Then, the needle was removed. The suture was pulled to introduce the distal end of the ileum into the colonic lumen. Then, the suture was knotted once on the colon again to fix the ileum to the colon. The incision in the proximal end of the colon was not closed. At the 2nd week after the operation, X-ray examinations demonstrated that the ileocolonic passages with no leakage at the anastomotic site were quite satisfactory. At the 4th week after the operation, there were no macroscopic or microscopic complications at the anastomotic site. The mucosal and serosal epithelia of the ileum and colon continued smoothly. This simple method may be very effective in preparing anastomosis in the gastrointestinal tract, especially in small laboratory animals for nutritional and surgical experiments.
机译:在大鼠中进行回肠结肠造口术的简单方法。盲肠附近的结肠被结扎,包括其动脉和静脉。盲肠的主动脉和静脉结扎。然后,切开盲肠。在结肠近端的反肠系膜侧切一个纵向切口,长约7-8毫米。将21-G针插入距离结肠反肠系膜侧近端2 cm处的切口。将尼龙缝合线打结到回肠的远端一次,并引入到先前已经穿过结肠的针尖中。然后,拔下针头。拉动缝线以将回肠的远端引入结肠腔。然后,将缝合线再次在结肠上打结以将回肠固定在结肠上。结肠近端的切口未闭合。术后第二周,X射线检查显示吻合口处未漏出的回结肠口非常满意。术后第4周,吻合部位无肉眼或镜下并发症。回肠和结肠的粘膜和浆膜上皮继续顺利进行。这种简单的方法在准备胃肠道吻合中可能非常有效,尤其是在用于营养和外科实验的小型实验动物中。

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