Battery of Tests for Profiling Abnormalities of Vitamin K-Dependent Coagulation Factors in Drug-Toxicity Studies in Rats

摘要

A battery of simple tests for profiling abnormalities of vitamin K-dependent coagulation factors encountered in drug-toxicity studies was verified in rats treated with warfarin (3 and 10 mg/kg, p.o). The thrombotest, or hepaplastin-test, is useful as a follow-up test after routine screening tests for coagulation abnormalities based on PT and APTT, to rule out other coagulation-factor abnormalities. Measurement of coagulation factor activities (factors Ⅱ, Ⅶ, Ⅸ and Ⅹ) using factor-deficient human plasmas provides direct evidence of decreased activities of vitamin K-dependent factors. Furthermore, Echis carinatus venom coagulation time, together with factor Ⅱ activity, allows us to confirm the generation of PIVKA-Ⅱ.