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SAMP1/Sku as a Murine Model for Tubulointerstitial Nephritis: a Study Using Unilateral Ureteral Obstruction

机译:SAMP1 / Sku作为肾小管间质性肾炎的小鼠模型:使用单侧输尿管梗阻的研究。

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The SAMP1/Sku mouse is a substrain of the SAMP1 (senescence-accelerated-mouse prone 1) which exhibits renal mononuclear cell infiltration from a younger age. We hypothesized that this renal characteristic is related to the incidence of tubulointerstitial nephritis (TIN). The purpose of the present study was to evaluate the applicability of the SAMP1/Sku mouse as a murine model for TIN. TIN was experimentally induced by unilateral ureteral obstruction (UUO). The SAMP1/Sku and control ICR of both sexesreceived either a sham or UUO operation and were sacrificed 7 days after the operation. The kidneys of the mice were observed histopathologically, immunohistochemically and semiquantitatively. UUO kidneys showed mononuclear cell infiltration, tubular atrophy and interstitial fibrosis. In males, semiquantitative scores of mononuclear cell infiltration, tubular atrophy, and F4/80, α-smooth muscle actin (α-SMA) and transforming growth factor (TGF)-β1 reactions were significantly higher in SAMP1/Sku than in ICR. Likewise, in females, tubular atrophy and F4/80 reaction scores were significantly higher in SAMP1/Sku than in ICR. In conclusion, induction of TIN damage by UUO was more serious in SAMP1/Sku mice than in ICR. Therefore, we propose that SAMP1/Sku mice, especially male SAMP1/ Sku, have congenital risk factors for the development of TIN.
机译:SAMP1 / Sku小鼠是SAMP1(衰老加速小鼠易感基因1)的亚型,从年轻时开始表现出肾单核细胞浸润。我们假设这种肾脏特征与肾小管间质性肾炎(TIN)的发生有关。本研究的目的是评估SAMP1 / Sku小鼠作为TIN鼠模型的适用性。 TIN是由单侧输尿管梗阻(UUO)实验性诱导的。 SAMP1 / Sku和对照ICR均接受假手术或UUO手术,并在手术后7天被处死。从组织病理学,免疫组织化学和半定量观察小鼠的肾脏。 UUO肾脏显示单核细胞浸润,肾小管萎缩和间质纤维化。在男性中,SAMP1 / Sku中单核细胞浸润,肾小管萎缩和F4 / 80,α平滑肌肌动蛋白(α-SMA)和转化生长因子(TGF)-β1反应的半定量得分均显着高于ICR。同样,在女性中,SAMP1 / Sku的肾小管萎缩和F4 / 80反应评分显着高于ICR。总之,与ICR相比,SAMP1 / Sku小鼠中UUO引起的TIN损伤更为严重。因此,我们建议SAMP1 / Sku小鼠,尤其是雄性SAMP1 / Sku,具有先天性TIN发生的危险因素。

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