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Re-Establishment of Complement C6-Deficient Rabbit Colony by Cryopreserved Sperm Transported from Abroad

机译:冷冻保存的精子从国外运来的补体C6缺陷兔集落的重建

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摘要

Introducing rabbits as genetic materials into institutes for experimental animals from other colonies is essential for biomedical research. Currently, it is inconvenient to transport live rabbits from abroad, since they suffer from stress, are prone to accidents and must be inspected, as well as endure quarantine during the often long journey. To overcome these limitations of live animals, we transported sperm cryopreserved in liquid nitrogen. Rabbit sperm was collected from complement C6-deficient rabbits in Germany and then transported to Japan using a dry-shipper containing liquid nitrogen. After thawing the frozen semen and artificial insemination (AI), eleven live pups were born. Subsequently, a homozygous C6-deficient rabbit colony was established.
机译:将兔子作为遗传材料引入其他殖民地的实验动物研究所对于生物医学研究至关重要。目前,从国外运输活兔子很不方便,因为它们承受着压力,容易发生事故,必须进行检查,并且在经常漫长的旅途中要进行隔离。为了克服活体动物的这些限制,我们运输了冷冻保存在液氮中的精子。从德国的C6缺陷型补体兔中收集兔精子,然后使用含有液氮的干托运人将其运到日本。解冻冷冻精液并进行人工授精(AI)后,出生了11只活仔。随后,建立了纯合的C6缺陷兔集落。

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