Germ-Line Contribution of Embryonic Stem Cells in Chimeric Mice: Influence of Karyotype and In Vitro Differentiation Ability

摘要

the effect of the karyotype and the ability to differentiate in vitro upon germ-line transmission by A3-1 embryonic stem (ES) cells in chimeric mice were examined. Germ- line transmission was confirmed in ES cells exhibiting 38/100 and more of the normal karyotype, but no chimeric mice and/or germ-line transmitters were observed regardless of the karyotype when the cystic embryoid body (CEB) was formed on day 8 and later in the suspension culture. Germ-line transmission of the ES cells was not significantly influenced by formation of the simple embryoid body (SEB).