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Detection of Viral RNA by Electron Microscopic In situ Hybridization (ISH-EM) in The Germinal Epithelium of Mice Infected with Encephalomyocarditis (EMC) Virus

机译:通过电子显微镜原位杂交(ISH-EM)检测感染脑心肌炎(EMC)病毒的小鼠生殖上皮中病毒RNA。

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Electron microscopic in situ hybridization (ISH-EM) was first applied to the detection of viral RNA in the germinal epithelium of mice inoculated i.p. with 10~5 plaque- forming units. mouse of the D variant of encephalomyocarditis virus (EMC-D). Signals of viral RNA were first detected in a small number of Sertoli cells showing mild degeneration at 2 days post inoculation, and 2 days later, they were also detected in germinal cells and spermatogonia when Sertoli cells showed prominent degeneration. The results clearly demonstrated that the first site of viral attack in the germinal epithelium was Sertoli cell in the case of EMC-D-induced mouse orchitis.
机译:电子显微镜原位杂交(ISH-EM)首先用于检测经腹膜内注射的小鼠生发上皮中的病毒RNA。具有10〜5个噬菌斑形成单位。脑心肌炎病毒D变体(EMC-D)的小鼠。病毒RNA的信号首先在接种后2天出现少量轻度变性的Sertoli细胞中检测到,而2天后,当Sertoli细胞表现出明显的变性时在生殖细胞和精原细胞中也检测到它们。结果清楚地表明,在EMC-D诱导的小鼠睾丸炎的情况下,生发上皮中病毒侵袭的第一个部位是支持细胞。

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