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Production of Chimeric Mice from Cryopreserved Blastocysts

机译:由冷冻保存的胚泡生产嵌合小鼠

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Mouse blastocysts derived from in vivo fertilization were cryopreserved by a simple vitrification method. Most of the cryopreserved blastocysts survived while demonstrating a normal morphology after warming (88.1/100). The rate of cryopreserved balstocysts which developed to term as normal young was 56.8 /100, and chimeric mice were also produced from cryopreserved blastocysts injected with ES cells. As a result, 3.4 /100 of the injected embryos developed to term as germ line chimeric mice.
机译:通过简单的玻璃化方法将体内受精的小鼠胚泡冷冻保存。冷冻保存的大多数胚泡在变暖后仍能正常存活(88.1 / 100)。发育成正常年轻人的冷冻保存的囊肿的发生率为56.8 / 100,嵌合小鼠也从注射了ES细胞的冷冻保存的胚泡中产生。结果,注入的胚胎中有3.4 / 100发育成生殖系嵌合小鼠。

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