Comparative Analysis of Human and Macaque Monkey CD4: Differences in Formaldehyde Lability and Conformation

摘要

Here we characterized macaque monkey CD4 by flow cytometry. The results showed that relatively lower fluorescence intensity was observed depending on the monoclonal antibodies (mAbs) used for staining; Leu-3a exhibited four-fold lower intensity than Nu-Th/I, and that formalehyde fixation dramatically reduced fluorescence intensity of macaque CD4+ cells stained with Leu-3a but not of human cells. Nu-Th/I is therefore preferable for the analysis of macaque CD4. Pretreatment of eight mAb inhibited the other mAb binding to human CD4.