DETERMINATION OF MICROCYSTINS IN CYANOBACTERIAL BLOOMS BY SOLID- PHASE MICROEXTRACTION-HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

摘要

A solid-phase microextraction(SPME)method was applied to study microcystin(MC)profiles in a natural Microcystis sp. Bloom in a freshwater pond in Guangzhou, China. Three dominant MC variants, namely MC-LR, MC-YR, and MC-RR, were quantified. Simultaneous study of their total, extracellular, and intracellular profiles was made possible using SPME coupled to high-performance liquid chromatography. The total and intracellular concentrations of MC-LR in the bloom were 8.67×10~-2 μg/ ml and 1.93 mg/g respectively.