首页> 外文期刊>Environmental toxicology and chemistry >CHARACTERIZATION OF VITELLOGENIN GENE EXPRESSION IN ROUND GOBY (NEOGOBIUS MELANOSTOMUS) USING A QUANTITATIVE POLYMERASE CHAIN REACTION ASSAY
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CHARACTERIZATION OF VITELLOGENIN GENE EXPRESSION IN ROUND GOBY (NEOGOBIUS MELANOSTOMUS) USING A QUANTITATIVE POLYMERASE CHAIN REACTION ASSAY

机译:定量聚合酶链反应法鉴定圆形虾虎鱼卵黄蛋白原基因的表达

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摘要

A growing concern over endocrine disruption in aquatic species has prompted the development of molecular assays to monitor environmental impacts. This study describes the development of quantitative polymerase chain reaction (qPCR) assays to characterize the expression of two vitellogenin (Vtg) genes in the invasive round goby (Neogobius melanostomus). Fragments from the 18SrRNA (housekeeping gene), Vtg II, and Vtg III genes were cloned and sequenced. The qPCR assays were developed to detect hepatic Vtg expression in goby. The assays detected induction of both Vtg genes in nonreproductive males following a two-week laboratory exposure to 17β-estradiol (≥1 mg/kg i.p. injection). The assays were applied to goby from Hamilton Harbour, Lake Ontario (Canada), including those from sites where feminization and intersex of goby has been documented. Both Vtg genes had significantly higher expression in females compared to males. Male reproductive goby adopt either parental or sneaker tactics; Vtg II expression was higher in sneaker than in parental males but parental and nonreproductive males did not differ from each other. The Vtg III expression was significantly higher in sneaker males followed by parental males and nonreproductive males, respectively. The Vtg II and III expression in nonreproductive males was elevated in the contaminated site with documented intersex. This assay provides an important tool for the use of an invasive species in monitoring endocrine disruption in the Great Lakes region.
机译:对水生物种内分泌干扰的日益关注促使分子测定法发展以监测环境影响。这项研究描述了定量聚合酶链反应(qPCR)分析方法的发展,以表征侵袭性虾虎鱼(Neogobius melanostomus)中两个卵黄蛋白原(Vtg)基因的表达。克隆并测序了来自18SrRNA(管家基因),Vtg II和Vtg III基因的片段。开发了qPCR检测方法来检测虾虎鱼肝Vtg表达。在实验室接受17β-雌二醇(≥1 mg / kg腹腔注射)两周的实验室测试中,该检测方法检测到非生殖雄性中两种Vtg基因均被诱导。该检测方法应用于来自安大略湖(加拿大)汉密尔顿港的虾虎鱼,包括已经记录了虾虎鱼女性化和两性的地点的虾虎鱼。与男性相比,两种Vtg基因在女性中的表达均显着较高。雄性虾虎鱼采取父母或球鞋战术;运动鞋中Vtg II的表达高于亲本男性,但亲本和非生殖男性之间没有差异。 Vtg III表达分别在运动鞋雄性,其次是亲本雄性和非生殖雄性中更高。非生殖雄性动物的Vtg II和III表达在受污染的雌雄之间被升高。该测定法提供了一种重要工具,可用于利用入侵物种监测大湖地区的内分泌破坏。

著录项

  • 来源
    《Environmental toxicology and chemistry》 |2010年第12期|p.2751-2760|共10页
  • 作者单位

    Department of Biology, McMaster University, 1280 Main Street W., Hamilton, Ontario L8S 4K1, Canada;

    Department of Biology, McMaster University, 1280 Main Street W., Hamilton, Ontario L8S 4K1, Canada;

    Department of Psychology, Neuroscience and Behaviour, McMaster University, Hamilton, Ontario L8S 4K1, Canada;

    Department of Psychology, Neuroscience and Behaviour, McMaster University, Hamilton, Ontario L8S 4K1, Canada;

    Department of Biology, McMaster University, 1280 Main Street W., Hamilton, Ontario L8S 4K1, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    gobiidae; hamilton harbour; biomarker; vitellogenin; endocrine disruption;

    机译:戈壁科汉密尔顿港生物标志物卵黄蛋白原内分泌干​​扰;
  • 入库时间 2022-08-17 13:32:25

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