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首页> 外文期刊>Environmental toxicology and pharmacology >Deoxynivalenol induces apoptosis in chicken splenic lymphocytes via the reactive oxygen species-mediated mitochondrial pathway
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Deoxynivalenol induces apoptosis in chicken splenic lymphocytes via the reactive oxygen species-mediated mitochondrial pathway

机译:脱氧雪腐酚通过活性氧介导的线粒体途径诱导鸡脾淋巴细胞凋亡

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摘要

We investigated the immunotoxicity and cytotoxicity of deoxynivalenol (DON), a mycotoxin, and the mechanism by which it induces apoptosis. Chicken splenic lymphocytes treated with 0-50 μg/mL DON for 48 h inhibited growth of splenic lymphocytes in a dose-dependent manner, as revealed by the Cell Counting Kit-8 (CCK-8) bioassay. Annexin V-fluorescein isothiocyanate staining indicated that the number of apoptotic and necrotic cells were significantly higher compared with the control (P < 0.01). DON treatment induced ROS accumulation, resulting in reduced mitochondrial transmembrane potential, as detected by flow cytometry and 2',7'-dichlorofluorescein acetate and rhodamine 123 labeling, respectively. Enzyme linked immunosorbent assays revealed that the concentrations of p53, Bax, Bak-1, and Caspase-3 increased with increasing DON concentration (P<0.05 or P<0.01), whereas the concentrations of Bcl-2 decreased (P < 0.01) compared with the control. These data suggest that DON induces apoptosis in splenic lymphocytes via a ROS-mediated mitochondrial pathway.
机译:我们调查了脱氧雪腐酚(DON),一种真菌毒素的免疫毒性和细胞毒性,以及它诱导凋亡的机制。如细胞计数试剂盒8(CCK-8)生物测定所揭示的,用0-50μg/ mL DON处理48小时的鸡脾淋巴细胞以剂量依赖的方式抑制了脾淋巴细胞的生长。 Annexin V-荧光素异硫氰酸酯染色表明,凋亡细胞和坏死细胞数量明显高于对照组(P <0.01)。 DON处理诱导ROS积累,导致线粒体跨膜电位降低,分别通过流式细胞仪和2',7'-二氯荧光素乙酸盐和若丹明123标记检测。酶联免疫吸附试验表明,p53,Bax,Bak-1和Caspase-3的浓度随DON浓度的增加而增加(P <0.05或P <0.01),而Bcl-2的浓度则降低(P <0.01)。与控制。这些数据表明DON通过ROS介导的线粒体途径诱导脾淋巴细胞凋亡。

著录项

  • 来源
    《Environmental toxicology and pharmacology》 |2015年第1期|339-346|共8页
  • 作者单位

    College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China,Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China;

    College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China,Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China,School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China;

    College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China,Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China;

    College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China,Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China;

    College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China,Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China;

    College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China,Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China;

    College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China,Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China;

    College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China,Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China;

    College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China,Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China;

    College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China,Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Deoxynivalenol; Apoptosis; Mitochondria; Reactive oxygen species;

    机译:脱氧雪腐烯醇;细胞凋亡;线粒体;活性氧;

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