Biodegradation of isopropanol in a three phase fixed bed bioreactor: start up and acclimation using a previously-enriched microbial culture

摘要

The aerobic biodegradation of high liquid phase concentrations of 2-propanol(LPA) by a previously enriched solvent- tolerant bacterial consortium within a 1.9 1 fed-batch three phase fixed bed bioreactor was investigated. Solvent concentrations of up to 7.9 g 1~-1 were investigated. Previously enriched solvent-tolerant bacterial cells were immobilised onto porous glass cylinders as a means of bioprocess intensification. Bioreactor start-up and acclimation was studied and acetone concentration tracked as an indicator of IPA utilization, as the sole carbon source within a minimal salts medium (MSM).