TYROSINASE EXTRACT FROM AGARICUS BISPORUS MUSHROOM AND ITS IN NATURA TISSUE FOR SPECIFIC PHENOL REMOVAL

摘要

Phenols are toxic pollutants found in industrial wastes imposing several risks to human health. Tyrosinase (EC 1.14.18.1) is an oxygenase oxyreductase found in several life forms, like the mushroom Agaricus bisporus. This enzyme is readily available from this fungal tissue leading to high activity extracts without extensive purification, thus suggesting its potential as a biocatalyst for applications involving biomodification of phenols or bioremediation of phenol-polluted waters. The purpose of this work was to employ a crude extract from the Agaricus bisporus mushroom and its biomass for the removal of phenol from polluted water. Experiments were carried out without pH control. The initial phenol concentration in all solutions was 100 mg l~(-1). Four enzymatic concentrations (50, 100, 200 and 400 U ml~(-1)) were tested. Reactions, with 200 U ml~(-1) and 400 U ml~(-1) enzymatic activity, led to 90% of phenol removal. Chitosan was used as a coagulant, but no significant improvement was observed. The in natura fungi was also able to remove 90% of phenol, demostrating its viability as a biocatalyst in bioremediation process.