• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >Dispersion and Stability Optimization of TiO_2 Nanoparticles in Cell Culture Media
【24h】

Dispersion and Stability Optimization of TiO_2 Nanoparticles in Cell Culture Media

机译：TiO_2纳米粒子在细胞培养基中的分散性和稳定性优化

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Accurate evaluation of engineered nanomaterial toxicity requires not only comprehensive physical-chemical character ization of nanomaterials as produced, but also thorough understanding of nanomaterial properties and behavior under conditions similar to those used for in vitro and in vivo toxicity studies. In this investigation, TiO_2 nanoparticles were selected as a model nanoparticle and bovine serum albumin |BSA| was selected as a model protein for studying the effect of protein-nanopartcle interaction on TiO_2 nanoparticle dispersion in six different mammalian, bacteria, and yeast cell culture media. Great improvement in TiO_2 dispersion was observed upon the addition of BSA, even though the degree of dispersion varied from medium to medium and phosphate concentration in the cell culture media was one of the key factors governing nanoparticle dispersion. Fetal bovine serum (FBS) was an effective dispersing agent for TiO_2 nanoparticles in all six media due to synergistic effects of its multiple protein components, successfully reproduced using a simple "FBS mimic" protein cocktail containing similar concentrations of BSA, y-globulin, and apo-transferrin.
机译：对工程纳米材料毒性的准确评估不仅需要对所​​生产纳米材料进行全面的物理化学表征，而且还需要在类似于用于体外和体内毒性研究的条件下，全面了解纳米材料的特性和行为。在这项研究中，选择TiO_2纳米颗粒作为模型纳米颗粒，并使用牛血清白蛋白| BSA |。选择了一种蛋白质作为模型蛋白质，用于研究蛋白质-纳米粒子相互作用对TiO_2纳米颗粒在六种不同的哺乳动物，细菌和酵母细胞培养基中的分散的影响。加入BSA后，即使介质之间的分散程度不同，并且细胞培养基中的磷酸盐浓度是控制纳米颗粒分散的关键因素之一，也可以观察到TiO_2分散性的显着改善。胎牛血清（FBS）由于其多种蛋白质成分的协同作用而成为所有六种介质中TiO_2纳米粒子的有效分散剂，使用含有相似浓度的BSA，γ-球蛋白和载铁蛋白。

著录项

  • 来源
    《Environmental Science & Technology》 |2010年第19期|p.7309-7314|共6页
  • 作者

    ZHAOXIA JI; XUE JIN; SAJI GEORGE; TIAN XIA; HUAN MENG; XIANG WANG; ELIZABETH SUAREZ; HAIYUAN ZHANG; ERIC M.V. HOEK; HILARY GODWIN; ANDRE E. NEL; JEFFREY I. ZINK;

  • 作者单位

    California NanoSystems Institute University of California at Los Angeles,Los Angeles, California 90095;

    rnDepartment of Civil & Environmental Engineering University of California at Los Angeles,Los Angeles, California 90095;

    rnCalifornia NanoSystems Institute University of California at Los Angeles,Los Angeles, California 90095 Department of Medicine-Division of NanoMedicine University of California at Los Angeles,Los Angeles, California 90095;

    rnCalifornia NanoSystems Institute University of California at Los Angeles,Los Angeles, California 90095 Department of Medicine-Division of NanoMedicine University of California at Los Angeles,Los Angeles, California 90095;

    rnCalifornia NanoSystems Institute University of California at Los Angeles,Los Angeles, California 90095 Department of Medicine-Division of NanoMedicine University of California at Los Angeles,Los Angeles, California 90095;

    rnCalifornia NanoSystems Institute University of California at Los Angeles,Los Angeles, California 90095 Department of Medicine-Division of NanoMedicine University of California at Los Angeles,Los Angeles, California 90095;

    rnCalifornia NanoSystems Institute University of California at Los Angeles,Los Angeles, California 90095 Department of Environmental Health Sciences University of California at Los Angeles,Los Angeles, California 90095;

    rnCalifornia NanoSystems Institute University of California at Los Angeles,Los Angeles, California 90095 Department of Medicine-Division of NanoMedicine University of California at Los Angeles,Los Angeles, California 90095;

    rnCalifornia NanoSystems Institute University of California at Los Angeles,Los Angeles, California 90095 Department of Civil & Environmental Engineering University of California at Los Angeles,Los Angeles, California 90095;

    rnCalifornia NanoSystems Institute University of California at Los Angeles,Los Angeles, California 90095 Department of Environmental Health Sciences University of California at Los Angeles,Los Angeles, California 90095;

    rnCalifornia NanoSystems Institute University of California at Los Angeles,Los Angeles, California 90095 Department of Medicine-Division of NanoMedicine University of California at Los Angeles,Los Angeles, California 90095;

    rnCalifornia NanoSystems Institute University of California at Los Angeles,Los Angeles, California 90095 Department of Chemistry and Biochemistry,University of California at Los Angeles,Los Angeles, California 90095;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 14:04:01

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号