Microarray detection and qPCR screening of potential biomarkers of Folsomia Candida (Collembola: Isotomidae) exposed to Bt proteins (Cry1Ab and Cry1Ac)

摘要

The impact of fir proteins on non-target arthropods is less understood than their effects on target organisms where the mechanism of toxic action is known. Here, we report the effects of two Bt proteins, CrylAb and CrylAc, on gene expression in the non-target collembolan, Folsomia Candida. A customized microarray was used to study gene expression in F. Candida specimens that were exposed to CrylAb and CrylAc. All selected transcripts were subsequently confirmed by qPCR. Eleven transcripts were finally verified, and three of them were annotated. The responses of all eleven transcripts were tested in specimens for both CrylAb and CrylAc at a series of concentrations. These transcripts were separated into two and three groups for CrylAb and CrylAc, respectively, depend on their expression levels. However, those eleven transcripts did not respond to the Bt proteins in fit-rice residues.