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Occurrence and seasonal variation of equine estrogens, equilin and equilenin, in the river water of Japan: Implication with endocrine- disrupting potentials to Japanese medaka (Oryzias latipes)

机译:在日本河水中马雌激素,马lin和马len素的发生和季节变化:对日本高med(Oryzias latipes)的内分泌干扰潜能

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摘要

In this study, we determined the concentration of equine estrogens, such as equilin (Eq) and equilenin (Eqn), in the river water collected from nine research stations in Hokkaido, Japan. The LC-MS/MS analysis revealed that Eq concentrations were 2.7 +/- 6.7, 0.22 +/- 0.12, and 1.2 +/- 0.64 ng/L in Sep 2015, Feb 2016, and Jul 2016, respectively. Eqn had concentration levels similar to those of Eq. Comparison of the concentrations at nine research stations showed that seasonal variation was observed in the detected Eq and Eqn concentration levels. This study was the first to show the occurrences and seasonal variation of Eq and Eqn in the river water of Japan. We further investigated the reproductive and transgenerational effects of Eq in Japanese medaka (Oryzias latipes) exposed to 10, 100, and 1000 ng/L for 21 days and assessed the transcriptional profiles of the estrogen-responsive genes in the livers of both sexes. The reproduction assay demonstrated that 1000 ng/L of Eq adversely affected the reproduction (i.e. fecundity) in the F-0 generation and that the hatching of F1 generation fertilized eggs was reduced in the 100 and 1000 ng/L treatment groups. Our qRT-PCR assay revealed that the mRNA expression levels of hepatic vitellogenin 1 and 2, choriogenin L and H, and estrogen receptor alpha were significantly up-regulated in males exposed to 100 and/or 1000 ng/L of Eq. In contrast, the transcriptional levels of several genes, such as pregnane X receptor and cytochrome P450 3A, were down-regulated in the livers of males after the 21-d exposure. These results suggest that Eq has endocrine-disrupting potential such as reproductive and transgenerational effects by the modulation of hepatic estrogen-responsive genes expression on medaka. (C) 2018 Published by Elsevier Ltd.
机译:在这项研究中,我们确定了从日本北海道的9个研究站收集的河水中马雌激素(例如马匹(Eq)和马匹素(Eqn))的浓度。 LC-MS / MS分析显示,2015年9月,2016年2月和2016年7月的Eq浓度分别为2.7 +/- 6.7、0.22 +/- 0.12和1.2 +/- 0.64 ng / L。方程式中的浓度水平与方程式中的相似。对9个研究站的浓度进行比较后发现,所检测到的Eq和Eqn浓度水平存在季节性变化。这项研究是第一个显示日本河流中Eq和Eqn的发生和季节变化的研究。我们进一步研究了当量10、100和1000 ng / L的日本21(Oryzias latipes)中的情商对21天的生殖和传代影响,并评估了男女肝脏中雌激素响应基因的转录谱。繁殖试验证明1000 ng / L的Eq对F-0代的繁殖(即繁殖力)产生不利影响,并且在100和1000 ng / L的治疗组中F1代受精卵的孵化率降低。我们的qRT-PCR分析显示,在暴露于100和/或1000 ng / L的Eq的男性中,肝卵黄蛋白原1和2,胆囊生成素L和H以及雌激素受体α的mRNA表达水平显着上调。相反,暴露于21天后,男性肝脏中的几个基因(如孕烷X受体和细胞色素P450 3A)的转录水平被下调。这些结果表明,Eq具有通过调节medaka上的肝雌激素反应性基因表达来破坏内分泌的潜力,例如生殖和转基因作用。 (C)2018由Elsevier Ltd.发布

著录项

  • 来源
    《Environmental Pollution》 |2018年第8期|281-288|共8页
  • 作者单位

    Ehime Univ, Grad Sch Agr, 3-5-7 Tarumi, Matsuyama, Ehime 7908566, Japan;

    Ariake Coll, Natl Inst Technol, Dept Creat Engn, 150 Higashi Hagio, Omuta, Fukuoka 8368585, Japan;

    Prefectural Univ Kumamoto, Fac Environm & Symbiot Sci, Higashi Ku, 3-1-100 Tsukide, Kumamoto 8628502, Japan;

    Prefectural Univ Kumamoto, Fac Environm & Symbiot Sci, Higashi Ku, 3-1-100 Tsukide, Kumamoto 8628502, Japan;

    Hirosaki Univ, Fac Agr & Life Sci, 3 Bunkyo Cho, Hirosaki, Aomori 0368561, Japan;

    Hokkaido Univ, Grad Sch Vet Med, Kita Ku, Kita 18,Nishi 9, Sapporo, Hokkaido 0600818, Japan;

    Hokkaido Univ, Field Sci Ctr Northern Biosphere, Shizunaimisono 111, Shinhidaka, Hokkaido 0560141, Japan;

    Ritsumeikan Univ, Dept Pharm, Coll Pharmaceut Sci, 1-1-1 Noji Higashi, Kusatsu, Shiga 5258577, Japan;

    Nagasaki Univ, Fac Environm Studies, 1-14 Bunkyo, Nagasaki 8528521, Japan;

    Ariake Coll, Natl Inst Technol, Dept Creat Engn, 150 Higashi Hagio, Omuta, Fukuoka 8368585, Japan;

    Prefectural Univ Kumamoto, Fac Environm & Symbiot Sci, Higashi Ku, 3-1-100 Tsukide, Kumamoto 8628502, Japan;

    Prefectural Univ Kumamoto, Fac Environm & Symbiot Sci, Higashi Ku, 3-1-100 Tsukide, Kumamoto 8628502, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Equine estrogen; LC-MS/MS; Reproduction; Transgeneration; Estrogen-responsive gene; Oryzias latipes;

    机译:马雌激素;LC-MS / MS;繁殖;转基因;雌激素反应基因;紫菜;
  • 入库时间 2022-08-17 13:25:51

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