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HEAVY METAL TOLERANCE IN CEROMOGENIC AND NON-CHROMOGENTIC MARINE BACTERIA FROM ARABIAN GULF

机译:阿拉伯湾生色和非生色海洋细菌的重金属耐受性

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摘要

A simple method - direct agar diffusion assay - was optimised for rapid assessment of heavy metal toxicity to marine chromogenic and non-chromogenic bacteria. The procedrue involved spotting of a 10 microliter test solution on the seeded agar plate and incubation of the plates at 30 deg C to accelerate bacterial growth. Under optimum conditions, test results were obtainable within 12-18 hr instead of 96 hr incubation time generlaly requried for a marine bacterial assay by conventional agar plate methods. A range of sixteen heavy metals, each at 5 different concentrations was tested.
机译:优化了一种简单的方法-直接琼脂扩散测定法-可快速评估重金属对海洋发色和非发色细菌的毒性。程序包括在接种的琼脂平板上点样10微升测试溶液,并在30℃下孵育平板以加速细菌生长。在最佳条件下,可在12至18小时内获得测试结果,而不是通常的琼脂平板法通常需要96小时的海洋细菌检测时间。测试了16种重金属,每种重金属含量分别为5种不同的浓度。

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