Modeling of Simultaneous Application of Vibrio sp (SK1) and Biochar Amendment for Removal of Pentachlorophenol in Soil

摘要

A combination of methods was applied in this study to evaluate the removal of pentachlorophenol (PCP) from an Indian agricultural soil. Treatment involved PCP-resistant microorganism Vibrio sp. (SK1); their activity was combined with the adsorbing capacity of biochar. First, PCP-resistant microorganism was used to transform PCP at batch level and further extended to farmland soil. Experimental results indicated that bacterial activity was found to have pronounced positive effect on PCP removal, and decreased the PCP level up to 75% of soil spiked with 100mg/kg, compared to control within 1-week period. Remaining PCP in soil was stabilized probably by sorption using biochar prepared from Pistia stratiotes; biochar was added at concentrations of 1%, 3%, and 5%. We observed that the 5% biochar strongly influenced the stabilization of PCP in the soil. 16s rRNA gene analysis indicated that bacterial strain belongs to Vibrio sp., which was not reported earlier for the PCP degradation. In our study, a parameter estimation method was used in mathematical models along with kinetic models. By using the nonlinear graphical regression method with the logistic growth curve, Monod, Contois, Moser, and the Tessier models, kinetic parameters were estimated.