Isolation and charactrerization of matural organic matter from lake water: two different adsorption chromatographic methods

摘要

Aqwuatic humic solutes were isolated from lake water by two different methods: non-ionic sorbing solid (XAD technique) and weakly basic anion exchanger (DEAE cellulose). The DEAE procedure removed from the fresh water sample at natural acidity 80/100 of the DOC as humic solutes. This was practically equal to the total amount of the three organic acids obtained directly by the XAD-8 resin at preadjusted acidity (pH 2) and of the acidic solutes obtained by a specific anion exchanger in connecntion with the XAD-8 column. The acidic gross character of the humic solutes obtained by the DEAE procedure was similar to the combined character of the four distinct acidic fractions obtained at pH 2 by different adsorbents.