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Transcriptomic analyses of tributyltin-induced sexual dimorphisms in rare minnow (Gobiocypris rarus) brains

机译:转录组学分析三丁基锡诱导的稀有min鱼(Gobiocypris rarus)大脑中的性二态性

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摘要

The brain of fish displays sexual dimorphisms and exhibits remarkable sexual plasticity throughout their life span. Although reproductive toxicity of tributyltin (TBT) in fish is well documented in fish, it remains unknown whether TBT interrupts sexual dimorphisms of fish brains. In this work, brain transcriptomic profiles of rare minnow (Gobiocypris rarus) was characterized and sex-biased genes were identified using RNA sequencing. Functional annotation and enrichment analysis were performed to reveal differences of gene products and pathways between the brains of male and female fish. Furthermore, transcriptomic responses of male and female brains to TBT at 10 ng/L were also investigated to understand effects of TBT on brain sexual dimorphisms. Only 345 male-biased and 273 female-biased genes were found in the brains. However, significant female-biased pathways of circadian rhythm and phototransduction were identified in the brains by enrichment analysis. Interestingly, following TBT exposure in the female fish, the circadian rhythm pathway was significantly disrupted based on enrichment analysis, while in the male fish, the phototransduction pathway was significantly disrupted. In the female fish, expression of genes (Per, Cry, Rev-Erb alpha, Ror, Dec and CKI delta/epsilon) in the circadian rhythm pathway was down-regulated after TBT exposure; while in the male fish, expression of genes (Rec, GNAT1_2, GNGTI, Rh/opsin, PDE and Arr) in the phototransduction pathway was up-regulated after TBT exposure. Overall, our results not only provide key data on the molecular basis of brain sexual dimorphisms in fish, but also offer valuable resources for investigating molecular mechanisms by which environmental chemicals might influence brain sexual plasticity.
机译:鱼的大脑在其整个生命周期中表现出性二态性,并表现出显着的性可塑性。尽管鱼类中的三丁基锡(TBT)的生殖毒性已有充分的文献记载,但尚不知道TBT是否会破坏鱼脑的性二态性。在这项工作中,对稀有min鱼(Gobiocypris rarus)的大脑转录组谱进行了表征,并使用RNA测序鉴定了性别偏向基因。进行功能注释和富集分析以揭示雄性和雌性鱼脑之间基因产物和途径的差异。此外,还研究了男性和女性大脑对10 ng / L的TBT的转录组反应,以了解TBT对脑性二态性的影响。在大脑中仅发现345个男性偏向基因和273个女性偏向基因。然而,通过富集分析在大脑中发现了明显的女性偏向的昼夜节律和光转导途径。有趣的是,在母鱼中进行TBT暴露后,基于富集分析,昼夜节律途径被显着破坏,而在雄鱼中,光转导途径被显着破坏。在雌性鱼类中,TBT暴露后,昼夜节律途径中的基因表达(Per,Cry,Rev-Erb alpha,Ror,Dec和CKIδ/ε)下调;在雄性鱼类中,TBT暴露后,光转导通路中的基因(Rec,GNAT1_2,GNGTI,Rh /视蛋白,PDE和Arr)的表达上调。总体而言,我们的研究结果不仅提供了鱼类脑性二态性的分子基础关键数据,而且为研究环境化学物质可能影响脑性可塑性的分子机制提供了宝贵的资源。

著录项

  • 来源
    《Ecotoxicology and Environmental Safety》 |2018年第7期|18-24|共7页
  • 作者单位

    Henan Univ Sci & Technol, Coll Anim Sci & Technol, Lab Aquat Environm & Anim Safety, Luoyang, Henan, Peoples R China;

    Henan Univ Sci & Technol, Coll Anim Sci & Technol, Lab Aquat Environm & Anim Safety, Luoyang, Henan, Peoples R China;

    Henan Univ Sci & Technol, Coll Anim Sci & Technol, Lab Aquat Environm & Anim Safety, Luoyang, Henan, Peoples R China;

    Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou, Hainan, Peoples R China;

    Henan Univ Sci & Technol, Coll Anim Sci & Technol, Lab Aquat Environm & Anim Safety, Luoyang, Henan, Peoples R China;

    Henan Univ Sci & Technol, Coll Anim Sci & Technol, Lab Aquat Environm & Anim Safety, Luoyang, Henan, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Tributyltin; Brain; Rare minnow; Circadian rhythm; Phototransduction; Sexual dimorphism;

    机译:三丁基锡;脑;稀有min鱼;昼夜节律;光转导;性二态性;
  • 入库时间 2022-08-17 13:22:37

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