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DNA damage and micronuclei in parthenogenetic and bisexual Darevskia rock lizards from the areas with different levels of soil pollution

机译:不同土壤污染地区单性和双性Darevskia蜥蜴的DNA损伤和微核

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Natural species are widely used as indicator organisms to estimate of the impact of environmental pollution. Here we present the results of first study of a reliability of parthenogenetic Darevskia armeniaca and bisexual Darevskia raddei rock lizards as sentinels for monitoring of environmental genotoxicity. The comet assay and micronucleus test were applied to the lizards sampled in six areas in Armenia and Artsakh with different levels of soil contamination. The results obtained showed a clear relationship between the pollution level of lizards' habitats and the frequency of DNA damage in the comet assay. Low baseline frequency of micronuclei in D. armeniaca and D. raddei, however, makes this parameter ineffective for environmental genotoxicity evaluation. The parthenogenetic lizards D. armeniaca showed higher sensitivity toward genotoxic pollutions compared with bisexual D. raddei living in the same environment. The correlations between soil content of heavy metals Cr, Cu, Zn, Mo, Pb and DNA damage in D. armeniaca and between Cu, As, Mo, Pb and DNA damage in D. raddei were revealed. Overall, the lizards D. raddei and D. armeniaca appeared to be sensitive species in detecting soil pollution in natural environment. The application of the comet assay in Darevskia lizard species can be considered as a more appropriate method than a micronucleus test. The use of parthenogenetic lizards D. armeniaca as bioindicator will permit to assess the environmental genotoxicity independent of the genetic polymorphism of bisexual species.
机译:天然物种被广泛用作指示生物,以估计环境污染的影响。在这里,我们介绍对雌性孤雌Darevskia armeniaca和双性恋Darevskia raddei蜥蜴作为监测环境遗传毒性的前哨的可靠性的首次研究结果。彗星试验和微核试验适用于亚美尼亚和阿尔萨克六个地区不同土壤污染程度的蜥蜴。所得结果表明,在彗星试验中,蜥蜴栖息地的污染程度与DNA损伤的频率之间存在明显的关系。 D. armeniaca和D. raddei中微核的低基线频率使该参数对于环境遗传毒性评估无效。单性生殖蜥蜴D. armeniaca与生活在同一环境中的双性恋拉德迪氏菌相比,对遗传毒性污染表现出更高的敏感性。揭示了土壤中重金属Cr,Cu,Zn,Mo,Pb的含量与D. armeniaca中DNA损伤之间的相关性,以及R. raddei中的Cu,As,Mo,Pb与DNA损伤之间的相关性。总体而言,蜥蜴D. raddei和D. armeniaca似乎是检测自然环境中土壤污染的敏感物种。比起微核试验,彗星试验在达里夫斯基蜥蜴物种中的应用可以被认为是更合适的方法。单性生殖蜥蜴D. armeniaca作为生物指示剂的使用将允许评估环境遗传毒性,而与双性恋物种的遗传多态性无关。

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