Role of Polymorphisms in the Interleukin-10 Gene in Determining Disease Susceptibility and Phenotype in Inflamatory Bowel Disease

摘要

Interleukin-10 (IL-10) has a key role in regulating mucosal inflammation. The role of functional polymorphisms at positions −627 and −1117 in the IL-10 gene as candidate susceptibility loci in inflammatory bowel disease and their importance in determining disease extent were evaluated in 159 patients with ulcerative colitis (83 left-sided; 76 extensive), 90 patients with Crohn's disease (22 small bowel; 29 large bowel; 39 both), and 227 controls. Genotyping was performed either by PCR-RFLP assays (−627 site) or SSCP analysis (−1117 site). An excess of −627A allele was observed in patients with left-sided colitis (52%) compared with controls (33%; P = 0.004) suggesting that IL-10 may influence the extent of the disease. These results were not replicated in a newly recruited group (N = 100) of patients with UC. We conclude that polymorphisms at −627 and −1117 sites in the IL-10 gene do not contribute to the susceptibility to IBD or determining the extent of the disease in our population.