Specific Gene Expression in Pancreatic β-Cells Cloning and Characterization of Differentially Expressed Genes

摘要

Identification and characterization of genes expressed preferentially in pancreatic β-cells will clarify the mechanisms involved in the specialized properties of these cells, as well as providing new markers of the development fo type 1 diabetes. Despite major efforts, relatively few β-cell-specific genes have been charac- terized. We applied representational difference analy- sis to identify genes expressed selectively in the pan- creatic β-cell line βTC1 compared with the pancreatic α-cell line αTC1 and isolated 26 clones expressed at higher levels in the β-cells than in the α-cells.