Rapid Impairment of Skeletal Muscle Glucose Transport/Phosphorylation by Free Fatty Acids in Humans

摘要

The initial effects of free fatty acids (FFAs) on glu- cose transport/phosphorylation were studied in seven healthy men in the presence of elevated (1.44±0.16 mmol/l), basal (0.35±0.06 mmol/l), and low (<0.01 mmol/l; control) plasma FFA concentrations (P<0.05 between all groups) during euglycemic-hyperinsulinemic clamps. Concentrations of glucose-6-phosphate (G-6-P), inorganic phosphate (P_I), phosphocreatine, ADP, and pH in calf muscle were measured every 3.2 min for 180 min by using 31~P nuclear magnetic resonance spec- troscopy.