Subtype-Selective Expression of the Five Somatostatin Receptors (hSSTR1-5) in Human Pancreatic Islet Cells: A Quantitative Double-Label Immunohistochemical Analysis

摘要

We have developed a panel o rabbit polyclonal antipeptide antibodies against the five human somato- statin receptor subtypes (hSSTR1-5) and used them to analyze the pattern of expression of hSSTR1-5 in nor- mal human islet cells y quantitative double-label con- focal fluorescence immunocytochemistry. All five hSSTR subtypes were variable expressed in islets. The number of SSTR immunopositive cells showed a rank order of SSTR1>SSTR5>SSTR2>-SSTR3>SSTR4. SSTR1 was strongly colocalized with insulin in all β- Cells. SSTR5 was also an abundant isotype, being colo- Calized in 87/100 of β-cells. SSTR2 was found in 46/100 of β Cells, whereas SSTR3 and SSTR4 were relatively Poorly expressed.