β-Cell Destruction in NOD Mice Correlates With Fas (CD95) Expression on β-Cells and Proinflammatory Cytokine Expression in Islets

摘要

A mechanism of autoimmune destruction of islet β-cells in type 1 diabetes has been proposed to be the binding of Fas ligand (FasL) on T-cells to Fas receptors on β-cells. We investigated this proposal by examining the expression of FasL and Fas on islet-infiltrating T-cells and β-cells in relation to β-cell destruction in a syn- geneic islet transplant model in NOD mice. Diabetic NOD mice were transplanted with syngeneic islets and Injected with complete Freund's adjuvant, which pre- Vented diabetes recurrence (nondestructive insulitis), And with phosphate-buffered saline, which did not (β-cell Destructive insulitis).