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A novel transcription factor UvCGBP1 regulates development and virulence of rice false smut fungus Ustilaginoidea virens

机译:一种新的转录因子UVCGBP1调节水稻假粉碎机Ustilaginoidea Virens的显影和毒力

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Ustilaginoidea virens , causing rice false smut (RFS) is an economically important ascomycetous fungal pathogen distributed in rice-growing regions worldwide. Here, we identified a novel transcription factor UvCGBP1 ( C utinase G -box b inding p rotein) from this fungus, which is unique to ascomycetes. Deletion of UvCGBP1 affected development and virulence of U. virens . A total of 865 downstream target genes of UvCGBP1 was identified using ChIP-seq and the most significant KEGG enriched functional pathway was the MAPK signaling pathway. Approximately 36% of target genes contain the AGGGG (G-box) motif in their promoter. Among the targets, deletion of UvCGBP1 affected transcriptional and translational levels of UvPmk1 and UvSlt2 , both of which were important in virulence. ChIP-qPCR, yeast one-hybrid and EMSA confirmed that UvCGBP1 can bind the promoter of UvPmk1 or UvSlt2 . Overexpression of UvPmk1 in the ?UvCGBP1-33 mutant restored partially its virulence and hyphae growth, indicating that UvCGBP1 could function via the MAPK pathway to regulate fungal virulence. Taken together, this study uncovered a novel regulatory mechanism of fungal virulence linking the MAPK pathway mediated by a G-box binding transcription factor, UvCGBP1.
机译:Ustilaginoidea Virens,导致水稻假粉末(RFS)是在全球稻米产区分布的经济上重要的载染力病原体。在这里,我们鉴定了来自该真菌的新型转录因子UVCGBP1(C utinase G -box B inding p Rotein),其是ascomycetes的独特之作。缺失UVCGBP1影响和美国VIREN的发育和毒力。使用芯片-SEQ共鉴定了UVCGBP1的865个下游靶基因,并且最重要的Kegg富集功能途径是MAPK信号通路。大约36%的靶基因在其启动子中含有AgGGG(G-Box)基序。在靶标中,缺失UVCGBP1受影响的转录和翻译水平的UVPMK1和UVSLT2,两者在毒力中都很重要。 CHIP-QPCR,酵母单杂交和EMSA证实,UVCGBP1可以结合UVPMK1或UVSLT2的启动子。 UVCGBP1-33突变体的UVPMK1的过度表达恢复部分毒力和菌丝生长,表明UVCGBP1可以通过MAPK途径起作用以调节真菌毒力。该研究占据了将Mapk途径介导的Mapk途径,UVCGBP1介导的MAPK途径的真菌毒力新的调节机制。

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