Phytochemical investigation, synthesis of gold nanoparticles of Punica granatum and their biological activities against the selected pathogenic microorganism

摘要

The current study means to determine the activity of Punica. granatum peel crude extract and gold nanoparticles against pathogenic microorganisms. Five bacterial and three fungal species were used. Fourier-transform infrared spectroscopy (FTIR) and Ultraviolet spectroscopy (UV) of peel extracts and gold nanoparticles (AuNPs) were carried out in a range of 400-4000 nm and 300- 700nm respectively. Results showed that P. granatum peel crude extract have medicinally important phytochemical constituents which have commercial importance to prepare drugs. Synthesized AuNPs were confirmed and characterized by FTIR and (UV-Vis) spectroscopy. Surface plasma resonance (SPR) peak in absorption spectra showed absorption at 525 nm by using (UV-Vis) spectroscopy. Bioactive compounds from the peel of P. granatum and extract mediated AuNPs can be significantly used as an alternate medicine to replace the available antibiotics, which have adverse side effect. P. granatum peel were selected instead of other parts because other parts were already screened and have been reported to contain many phytochemical ingredients, making them pharmacologically valuable.