The optimum range of temperature for germination (96-100%) of Calamintha cretica , an herb with potential pharmaceutical and horticultural uses, was 15 to 20 °C, with 10 and 30 °C cardinal temperatures. Storage up to one year did not affect germination. The effect of zeatin (ZEA), 6-benzyladenine (BA), kinetin, and 6-γ-γ-(dimethylallylamino)-purine added in MS medium at concentrations from 0.0 to 8.0 mg L -1 was tested for shoot proliferation of both adult- and seedling-origin nodal explants at first- and sub-culture. Both explant types responded similarly during in vitro culture. At cytokinin concentrations up to 1 mg L -1 explant response was high (over 85%) but shoot number per explant was low (1.2-2.2). Increasing cytokinin from 2.0 to 8.0 mg L -1 resulted to an analogous decrease of explant response and shoot length, and an increase of shoot number, particularly when ZEA or BA was used (5.0-6.6 shoots per explant, 0.5-1.0 cm long) with simultaneous though increase of hyperhydricity (up to 50%). The addition of 0.1 mg L -1 naphthaleneacetic acid into the 8.0 mg L -1 BA medium almost eliminated hyperhydricity and increased explant response, while the increase of agar concentration from 8.0 to 12.0 g L -1 eliminated hyperhidricity and induced the highest shoot proliferation (93-95% explant response, 11.2-12.3 shoots per explant, 0.8-1.0 cm long). Microshoots and microshoot clusters rooted (88-96%) on half-strength MS medium either hormone free or supplemented with 1 to 4 mg L -1 indole-3-butyric acid. Plantlets survived at 80% to 100% after ex vitro acclimatization in peat: perlite 1:1 (v/v).
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机译:萌发的最佳温度范围(96-100%)Calamintha Cretica,一种具有潜在药物和园艺用途的草本植物,为15至20℃,具有10至30°C的红线温度。存储最多一年不会影响发芽。在0.0至8.0mgL -1的浓度下,在MS培养基中加入的Zeepin(Zea),6-苄基腺嘌呤(Ba),kinetin和6-γ-γ-(二甲基酰胺酰胺氨基) - 对两者的次次增殖进行测试成人和幼苗起源在第一和子文化中的核心植物。在体外培养期间,两种外植体类型都类似地响应。在Cytokinin浓度高达1mg L -1外植体反应(超过85%),但每种外植体的枝条低(1.2-2.2)。从2.0到8.0 mg的L -1增加细胞素素导致外植体响应和射击长度的类似减少,以及射击数量的增加,特别是当使用Zea或Ba时(每次外部植物的5.0-6.6芽,0.5-1.0厘米长)同时增加高级性(高达50%)。将0.1mg L -1萘酰基乙酸加入8.0mg L -1 BA培养基,几乎消除了高液化和增加的外植体反应,而琼脂浓度的增加从8.0至12.0g l -1消除了纯度,并诱导了最高的芽增殖( 93-95%的外植体响应,11.2-12.3每次外植体的芽,长0.8-1.0厘米)。 MicroShoots和MicroShoot簇生根(88-96%)在半强度MS培养基上自由或补充1至4mg L -1吲哚-3-丁酸的激素。在泥煤中的exverro适应后,Plantlets在80%至100%中存活:珍珠岩1:1(v / v)。
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