An appropriate analytical technique for determination of kaempferol (KMF) by its perturbation effecton Belousov-Zhabotinskii (B-Z) oscillating system was reported. The macrocyclic copper (II) complex[CuL] (ClO4)2] was used as a catalyst while malic acid as substrate in B-Z system. The ligand L in themacrocyclic-complex is 5, 7, 7, 12, 14, 14-hexamethyl-1, 4, 8, 11-tetraazacyclotetradeca-4, 11-diene.Experimental outcomes shown the perturbation effect of KMF could cause the change in the amplitudeof oscillation (?A) which was directly related to its concentration (2.5 × 10-6to 3.0 × 10-4 mol/L) withcorrelation coefficient of 0.99315. The calculated relative standard deviation (RSD) is 3.1 % by eightsamples (1.8 × 10-5 mol/L) and the observed lower limit of detection is 1.25 × 10-6 mol/L. The cyclicvoltammetry (CV) experiments were used to confirm the redox reaction between kaempferol andsodium bromate. Furthermore, the reaction perturbation mechanism was derived from the well-knownFKN (Field-Koros-Noyes) oscillation mechanism.
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