EFFECTS OF COLCHICINE TREATMENT ON THE MICROTUBULE CYTOSKELETON AND TOTAL PROTEIN DURING MICROSPOROGENESIS IN GINKGO BILOBA L.

摘要

The purpose of this study was to examine the effects of colchicine treatment on the microtubule cytoskeleton andthe expression of proteins during microsporogenesis in G. biloba, as observed by immunofluorescence and 2-DE analysisin microsporangia treated with colchicine. The results showed the microtubule structures were affected by the colchicinein Ginkgo biloba, but the treatment effect of the colchicine had certain limitation in G. biloba. The percentage ofmicrosporocytes whose microtubule structures were affected by the colchicine treatment was less than that observed inother plant species, not higher than 10 %. It was also found that the expression level of several endogenous proteins werechanged in G. biloba when the microsporangia were treated with colchicine. Although we only tested colchicines wasonly tested in the present study, G. biloba appeared to possess factors that restricted the effect of such chemical agents.Our observations led us to speculate that the endogenous proteins are possibly responsible for the reduced effects ofcolchicine treatment in G. biloba.