首页> 外文期刊>Journal of Clinical Microbiology >Bovine adenovirus type 10 identified in fatal cases of adenovirus-associated enteric disease in cattle by in situ hybridization.
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Bovine adenovirus type 10 identified in fatal cases of adenovirus-associated enteric disease in cattle by in situ hybridization.

机译:通过原位杂交在牛的腺病毒相关肠道疾病的致命病例中鉴定出10型牛腺病毒。

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A severe, naturally occurring enteric disease of cattle in which adenovirus inclusions are present in the intestinal vascular endothelium has been recognized in several countries; three different adenovirus serotypes have been isolated from affected animals. An in situ hybridization technique for the detection of bovine adenoviral DNA was developed and applied to tissue from 13 cattle in Northern Ireland diagnosed to have the disease. Bovine adenovirus serotype 10 (BAV-10) was identified in the vascular inclusions of all cattle, providing strong evidence that adenoviral enteric vascular disease in cattle is associated with this serotype. The existence of BAV-10 has only recently been recognized. The first molecular biology-based technique for the diagnosis of BAV-10 infection is described. The animals in the present study are the first in which BAV-10 has had a confirmed role in a pathologic process.
机译:在一些国家已经认识到一种严重的,自然发生的牛肠道疾病,在肠道血管内皮中存在腺病毒夹杂物。从患病动物中分离出三种不同的腺病毒血清型。开发了一种用于检测牛腺病毒DNA的原位杂交技术,并将其应用于北爱尔兰的13头被诊断患有该病的牛的组织。在所有牛的血管内含物中均鉴定出牛腺病毒血清型10(BAV-10),提供了有力的证据表明牛的腺病毒肠血管疾病与该血清型有关。 BAV-10的存在直到最近才被发现。描述了第一个基于分子生物学的BAV-10感染诊断技术。本研究中的动物是第一批BAV-10在病理过程中具有确定作用的动物。

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