Characterization of C. elegans RING finger protein 1, a binding partner of ubiquitin-conjugating enzyme 1

Emily Crowe; E.Peter M Candido

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Characterization of C. elegans RING finger protein 1, a binding partner of ubiquitin-conjugating enzyme 1

机译：秀丽隐杆线虫无名指蛋白1（泛素结合酶1的结合伴侣）的表征

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Inayeasttwo-hybridscreen,RINGfingerprotein1(RFP-1)andUBR1wereidentifiedaspotentialbindingpartnersofemC.elegans/emUBC-1,aubiquitin-conjugatingenzymewithahighdegreeofidentitytoemS.cerevisiae/emUBC2/RAD6.TheinteractionofRFP-1andUBC-1wasconfirmedbyco-immunoprecipitationexperiments.YeastinteractiontrapexperimentsmappedtheregionofinteractiontothebasicN-terminal313residuesofRFP-1.Theacidiccarboxy-terminalextensionofUBC-1wasnotrequiredfortheinteractionwithRFP-1.WesternblotanalysisandindirectimmunohistochemicalstainingshowthatRFP-1ispresentinembryos,larvae,andadults,whereitisfoundinintestinal,nervering,pharyngeal,gonadal,andoocytecellnuclei.Double-strandedRNAinterferenceexperimentsagainstemrfp-1/emindicatethatthisgeneisrequiredforL1development,vulvaldevelopment,andforegglaying.Bycontrast,RNAinterferenceagainstemubc-1/emgavenoobviousphenotype,suggestingthatemubc-1/emisnonessentialorisfunctionallyredundant./p/div

机译：在两次双杂交筛选中，鉴定了环状指蛋白1（RFP-1）和UBR1作为秀丽隐杆线虫UBC-1，与泛素结合酶具有高度同源性的潜在结合伴侣，它们与酿酒酵母 UBC2 / RAD6具有高度的同源性。 YeastinteractiontrapexperimentsmappedtheregionofinteractiontothebasicN-terminal313residuesofRFP-1.Theacidiccarboxy-terminalextensionofUBC-1wasnotrequiredfortheinteractionwithRFP-1.WesternblotanalysisandindirectimmunohistochemicalstainingshowthatRFP-1ispresentinembryos，幼虫，andadults，whereitisfoundinintestinal，nervering，咽，性腺，andoocytecellnuclei.Double-strandedRNAinterferenceexperimentsagainst RFP-1 indicatethatthisgeneisrequiredforL1development，vulvaldevelopment，andforegglaying 。相反，RNA干扰 ubc-1 gavenoo明显表型，暗示 ubc-1 在功能上是多余的。 展开▼

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《Developmental biology》 |2004年第2期|共页

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Emily Crowe; E.Peter M Candido;
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中图分类生物科学;

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1. Characterization of C-elegans RING finger protein 1, a binding partner of ubiquitin-conjugating enzyme 1 [J] . Crowe E., Candido EPM. Developmental biology . 2004,第2期

机译：C-线虫无名指蛋白1（泛素结合酶1的结合伴侣）的表征

2. The LIM domain protein UNC-95 is required for the assembly of muscle attachment structures and is regulated by the RING finger protein RNF-5 in C. elegans [J] . Limor Broday, Irina Kolotuev, Christine Didier, Journal of cell biology . 2004,第6期

机译：LIM域蛋白UNC-95是肌肉附着结构组装所必需的，并由秀丽隐杆线虫中的RING指蛋白RNF-5调节。

3. The conserved transmembrane RING finger protein PLR-1 downregulates Wnt signaling by reducing Frizzled, Ror and Ryk cell-surface levels in C. elegans [J] . MoffatL.L., RobinsonR.E., BakoulisA., Development . 2014,第3期

机译：保守的跨膜RING指蛋白PLR-1通过降低秀丽隐杆线虫的卷曲，Ror和Ryk细胞表面水平来下调Wnt信号传导。

4. Measuring Proteins Across Developmental Stages of C. elegans Using Molecular Weight Fractionation and High-resolution Mass Spectrometry [C] . Gennifer E. Merrihew, Vagisha Sharma, James R. Hibbard, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：使用分子量分级和高分辨率质谱法测量杆状杆菌的发育阶段的蛋白质

5. Molecular characterization of poxviral RING finger proteins: Virosome localization and identification of DNA binding and apoptosis inhibition activity. [D] . Brick, David Joseph. 2001

机译：痘病毒RING手指蛋白的分子表征：病毒体定位以及DNA结合和凋亡抑制活性的鉴定。

6. Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair [O] . Helle D. Ulrich, Stefan Jentsch 2000

机译：两种RING指蛋白介导泛素结合酶在DNA修复中的协同作用

7. Characterization of C. elegans RING finger protein 1, a binding partner of ubiquitin-conjugating enzyme 1 [O] . Crowe Emily, Candido E.Peter M 2004

机译：秀丽隐杆线虫无名指蛋白1的表征，泛素缀合酶1的结合伴侣

1. UEV蛋白,一个保守的类泛素结合酶蛋白家族 [J] . 冯强 ,冯朝晖 . 癌变．畸变．突变 . 1999,第003期

2. 胆囊癌患者胆囊癌组织泛素结合酶E2 T蛋白表达及其临床意义探讨 [J] . 于涛 ,李奎武 ,姚琳 . 实用肝脏病杂志 . 2021,第004期

3. ISG15蛋白泛素结合酶UBCH6的原核表达及多克隆抗体制备 [J] . 李玉霄 ,唐榕泽 ,高跃美 . 动物医学进展 . 2020,第012期

4. 普通油茶泛素结合酶UBE2-J2的cDNA序列及蛋白质结构分析 [J] . 林萍 ,姚小华 ,曹永庆 . 林业科学研究 . 2013,第006期

5. 鸽泛素结合酶蛋白(UBE2)基因的生物信息学分析 [J] . 高鹏飞 ,赵慧婷 ,王钦德 . 畜牧兽医科技信息 . 2010,第011期

6. 普通油茶泛素结合酶UBE2-J2的cDNA序列及蛋白质结构分析 [C] . LIN Ping ,林萍 ,YAO Xiao-hua . 全国油茶技术协作组第一届油茶学术交流会 . 2013

7. 稻瘟病菌过氧化物酶体泛素结合酶PEX4及其锚定蛋白PEX22基因双敲除及突变体表型分析 [A] . 张柯 . 2018

1. 一种兔抗黄瓜泛素结合酶CsE2蛋白多克隆抗体及其制备方法 [P] . 中国专利： CN105018437A . 2015-11-04

2. 泛素结合结构域融合蛋白在泛素化蛋白质的检测和分离中的应用 [P] . 中国专利： CN103059141A . 2013-04-24

3. GDP dissociation stimulating protein, brain-specific nucleosome assembly protein, skeletal muscle specific ubiquitin-conjugating enzyme, cell proliferation protein, phosphatidylinositolkinase, nel related proteins [P] . 外国专利： EP1295944B1 . 2007-05-16

机译：GDP解离刺激蛋白，脑特异性核小体组装蛋白，骨骼肌特异性泛素结合酶，细胞增殖蛋白，磷脂酰肌醇激酶，内尔相关蛋白

4. GDP dissociation stimulating protein, brain-specific nucleosome assembly protein, skeletal muscle specific ubiquitin-conjugating enzyme, cell proliferation protein, phosphatidylinositolkinase, nel related proteins [P] . 外国专利： EP1295944A3 . 2004-04-21

机译：GDP解离刺激蛋白，脑特异性核小体组装蛋白，骨骼肌特异性泛素结合酶，细胞增殖蛋白，磷脂酰肌醇激酶，内尔相关蛋白

5. GDP dissociation stimulating protein, brain-specific nucleosome assembly protein, skeletal muscle specific ubiquitin-conjugating enzyme, cell proliferation protein, phosphatidylinositolkinase, nel related proteins [P] . 外国专利： EP1295944A2 . 2003-03-26

机译：GDP解离刺激蛋白，脑特异性核小体组装蛋白，骨骼肌特异性泛素结合酶，细胞增殖蛋白，磷脂酰肌醇激酶，内尔相关蛋白

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Characterization of C. elegans RING finger protein 1, a binding partner of ubiquitin-conjugating enzyme 1

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