RNA synthesis and coding capacity of polyadenylated and nonpolyadenylated mRNA from cultures of differentiating Drosophila melanogaster myoblasts☆

摘要

WehaveinvestigatedthesynthesisandcodingcapacityofRNAisolatedfromculturesofdifferentiatingDrosophilaembryonicmusclecells.Wefindthatfollowingmusclecellfusion,thesedimentationprofileofnewlysynthesizedpolyadenylatedRNAbecomessomewhatlighter.Invitrotranslationproductsanalyzedbytwo-dimensionalgelelectrophoresisindicatethatthecodingcapacityoftranslatablemyogenicmRNAchangesduringdifferentiation.Agroupofseveralmuscle-specificproteins(includingthecontractileproteins)istranslatedonlyfrommRNAisolatedaftertheinitiationoffusion.Thispatterncoincideswithproteinssynthesizedinvivoduringdifferentiation.Additionally,wefindthatpolyadenylatedandnonpolyadenylatedmyogenicmRNAfromagivendevelopmentalstageinculturehaveextremelysimilarcodingpotentials.