Chromophores of allophycocyanin and R-phycocyanin

摘要

pThe biliprotein allophycocyanin was purified from iPhormidium luridum, Anabaena variabilis/i and iPlectonema boryanum/i. R-phycocyanin was purified from iRhodymenia palmata/i. The chromophores were cleaved from the denatured protein by methanol hydrolysis. They were purified and crystallized as the dimethyl esters. Chromatographic and absorption-spectral (visible–ultraviolet and infrared) comparisons with reference material have established phycocyanobilin as the chromophore of allophycocyanin. Phycocyanobilin and phycoerythrobilin were shown to be the chromophores of R-phycocyanin./p