Production of different cytokines, at the level of the singular cell, was studied. MNC isolated from synovial fluid and peripheral blood were tested for the spontaneous production of IL1α, IL1β, IFNγ, IL-2, IL-4, IL-5, IL-6, IL-10, TNFα. and GM-CSF. All patients (20), fulfilled the ARA criteria for the diagnosis of JRA. MNC from peripheral blood from 24 healthy donors were used like controls. After stimulation with FMA+Ionomycin at sub optimal periods of stimulation, the following cytokines were analyzed: after 45': IFNγ, IL-2, and TNFα; after 2 hs: IL-10, IL-4, GM-CSF. The results showed that there is a spontaneous production of TNFα in MNC from SF, in JRA patients. TNFα was present in the patients with higher ESR and higher white cell count in SF. After stimulation, production of IFNγ was significantly lower in the JRA patients(p=.0009). Conversely, IL-10 production was significantly higher than in the control group(p=.0013). Our results suggest the presence of “primed” T cells in synovial fluid. Also, it seems that T cells from SF have a tendency to follow the pattern Th1-Th2 (regarding cytokine production).
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