Preparation of Ru(ii)@oligonucleotide nanosized polymers as potential tumor-imaging luminescent probes

摘要

The development of Ru( II ) complexes as luminescent probes has attracted increasing attention in recent decades. In this study, the nanosized polymers of two Ru( II ) complexes [Ru(phen) _(2) (dppz)](ClO _(4) ) _(2) ( 1 , phen = 1,10-phenanthrolin; dppz = dipyrido[3,2- a :2′,3′- c ]phenazine) and [Ru(phen) _(2) (Br-dppz)](ClO _(4) ) _(2) ( 2 , Br-dppz = 11-bromodipyrido[3,2- a :2′,3′- c ]phenazine) with oligonucleotides were prepared and investigated as potential tumor-imaging probes. The formation of the nanosized polymers, which had an average width of 125–438 nm and an average height of 3–6 nm, for 1 and 2 @oligonucleotides were observed through atomic force microscopy. The emission spectra indicated that the luminescence of 1 and 2 markedly increased after binding to oligonucleotides and double-strand DNA (calf thymus DNA), respectively. Moreover, further studies indicated that 1 @oligonucleotides and 2 @oligonucleotides can easily enter into tumor cells and selectively highlight the tumor area in the zebrafish bear xenograft tumor (MDA-MB-231). In summary, this study demonstrated that 1 @oligonucleotides and 2 @oligonucleotides could be developed as potential tumor-imaging luminescent probes for clinical diagnosis and therapy.