首页> 外文期刊>FEBS Letters >Molecular cloning, characterization and evolution of the gene encoding a new group of short‐chain α‐neurotoxins in an Australian elapid, Pseudonaja textilis
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Molecular cloning, characterization and evolution of the gene encoding a new group of short‐chain α‐neurotoxins in an Australian elapid, Pseudonaja textilis

机译:澳大利亚弹性体假单胞菌中编码一组新的短链α-神经毒素的基因的分子克隆,表征和进化

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>The structure and organization of five genes responsible for the synthesis of six isoforms of short-chain α-neurotoxins in Pseudonaja textilis venom are reported in this paper. This also forms the first report which describes the synthesis of two neurotoxin mRNA variants from one of these genes (Pt-sntx1) as a result of alternative splicing. Each gene consists of three exons which are separated by two introns and each has a functional promoter. The promoter activity was confirmed by both CAT assay and Real-Time PCR. A transcription initiation site, two putative TATA boxes, one CCAAT box and the transcription factor binding consensus sites for AP-1, GATA-2, c/EBPb were identified in the 5′ non-coding region of each gene. Phylogenetic analysis showed that these five genes from P. textilis constituted a distinct group which has evolved by gene duplication followed by accelerated evolution from an ancestral gene.
机译:>报道了五个基因的结构和组织,这些基因负责合成 Pseudonaja textilis 毒液中的短链α-神经轮蛋白的六个同工型。这也形成了第一个报告,该报告描述了由于选择性剪接而从这些基因之一( Pt-sntx1 )合成了两个神经毒素mRNA变体。每个基因由三个外显子组成,外显子被两个内含子隔开,每个外显子都有一个功能性启动子。通过CAT测定法和实时PCR确认了启动子活性。在每个基因的5'非编码区中确定了一个转录起始位点,两个推定的TATA盒,一个CCAAT盒和AP-1,GATA-2,c / EBPb的转录因子结合共有位点。系统发育分析表明,这五个基因来自 P。 textilis 构成了一个独特的群体,该群体通过基因复制和随后的祖先基因进化而进化。

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