The Complete Structure of the Core Oligosaccharide from Edwardsiella tarda EIB 202 Lipopolysaccharide

摘要

The chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharide of pathogenic Edwardsiella tarda strain EIB 202 were studied for the first time. The complete gene assignment for all LPS core biosynthesis gene functions was acquired. The complete structure of core oligosaccharide was investigated by 1 H and 13 C nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry MS n , and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. The following structure of the undecasaccharide was established: The heterogeneous appearance of the core oligosaccharide structure was due to the partial lack of β- d -Gal p and the replacement of α- d -Glc p NAcGly by α- d -Glc p NGly. The glycine location was identified by mass spectrometry.