首页> 外文期刊>BMC Genomics >In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes for Bapx1 and its co-regulation with Sox9 in the mammalian vertebral column
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In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes for Bapx1 and its co-regulation with Sox9 in the mammalian vertebral column

机译:对多种组织的体内全基因组分析确定了Bapx1的基因调控网络,新功能和下游调控基因,以及它与哺乳动物脊柱中Sox9的共同调控

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Background Vertebrate organogenesis is a highly complex process involving sequential cascades of transcription factor activation or repression. Interestingly a single developmental control gene can occasionally be essential for the morphogenesis and differentiation of tissues and organs arising from vastly disparate embryological lineages. Results Here we elucidated the role of the mammalian homeobox gene Bapx1 during the embryogenesis of five distinct organs at E12.5 - vertebral column, spleen, gut, forelimb and hindlimb - using expression profiling of sorted wildtype and mutant cells combined with genome wide binding site analysis. Furthermore we analyzed the development of the vertebral column at the molecular level by combining transcriptional profiling and genome wide binding data for Bapx1 with similarly generated data sets for Sox9 to assemble a detailed gene regulatory network revealing genes previously not reported to be controlled by either of these two transcription factors. Conclusions The gene regulatory network appears to control cell fate decisions and morphogenesis in the vertebral column along with the prevention of premature chondrocyte differentiation thus providing a detailed molecular view of vertebral column development.
机译:背景技术脊椎动物器官发生是一个高度复杂的过程,涉及转录因子激活或抑制的顺序级联。有趣的是,单个发育控制基因有时可能是由极为不同的胚胎谱系产生的组织和器官的形态发生和分化所必需的。结果在这里,我们利用分类的野生型和突变细胞的表达谱,结合基因组范围广泛的结合位点,阐明了哺乳动物同源异型盒基因Bapx1在E12.5五个不同器官的胚胎发生过程中的作用-脊柱,脾,肠,前肢和后肢分析。此外,我们通过结合转录谱和Bapx1的基因组范围结合数据与类似生成的Sox9数据集,在分子水平上分析了脊柱的发育,从而建立了详细的基因调控网络,揭示了先前未被报道受这两个基因控制的基因。两个转录因子。结论基因调控网络似乎可以控制椎骨细胞的命运决定和形态发生,并防止软骨细胞过早分化,从而为椎骨发育提供了详细的分子观点。

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