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Mast cell stabilization promotes antinociceptive effects in a mouse model of postoperative pain

机译:肥大细胞稳定在小鼠术后疼痛模型中促进镇痛作用

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Background: Nerve injury and consequent inflammatory responses produced by surgical incision result in a complicated pain status which still affects half of all surgical patients. Therefore, it is essential for anesthesiologists to identify the mechanisms of postoperative pain. Mast cells are resident cells of connective tissue and the mucosa that participate in the immune response. Degranulation of mast cells is involved in the development of postoperative pain and can be induced by surgical incision. The aim of this study was to investigate whether stabilization of mast cells causes an antinociceptive effect in a mouse model of postoperative pain.Methods: Postoperative pain was induced by making an incision in the hind paw of BALB/c mice. The mast cell membrane stabilizer cromoglycate (200 μg/20 μL) was injected before incision of the paw, and postoperative pain responses were measured by assessing guarding behavior, withdrawal threshold to mechanical stimuli, and latency of heat pain behavior 1, 2, and 7 days after the incision.Results: The incision produced guarding pain, mechanical allodynia, and heat hypersensitivity. Cromoglycate decreased the guarding pain score (day 1) and the withdrawal threshold to mechanical stimuli (days 1, 2, and 7). However, the withdrawal latency to heat was not affected by cromoglycate treatment.Conclusion: Cromoglycate significantly attenuated the pain response expressed as guarding pain and mechanical allodynia in a mouse model of postoperative pain. Thus, mast cell activation is likely a mechanism of postoperative pain and is an interesting target for the development of new therapies.
机译:背景:手术切口产生的神经损伤和随之而来的炎症反应导致复杂的疼痛状态,仍然影响着所有手术患者的一半。因此,对于麻醉医师来说,确定术后疼痛的机制至关重要。肥大细胞是参与免疫反应的结缔组织和粘膜的常驻细胞。肥大细胞的去颗粒化与术后疼痛的发展有关,并且可以通过手术切口来诱导。这项研究的目的是研究肥大细胞的稳定化是否在小鼠术后疼痛模型中产生抗伤害感受的作用。方法:通过在BALB / c小鼠后爪上切开切口来诱导术后疼痛。在足爪切开之前注射肥大细胞膜稳定剂色甘酸(200μg/ 20μL),并通过评估保护行为,对机械刺激的戒断阈值和热痛行为1、2和7的潜伏期来测量术后疼痛反应结果:切口产生保护性疼痛,机械性异常性疼痛和热超敏反应。血糖浓度降低了保护性疼痛评分(第1天)和对机械刺激的戒断阈值(第1、2和7天)。然而,降糖的潜伏期不受糖基葡萄糖酸盐治疗的影响。结论:糖基葡萄糖酸盐显着减轻了术后疼痛小鼠模型中表现为保护性疼痛和机械性异常性疼痛的疼痛反应。因此,肥大细胞活化可能是术后疼痛的机制,并且是开发新疗法的有趣目标。

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