Protective effect of calcium dobesilate combined with benazepril therapy on renal injury in patients with early diabetic nephropathy and the possible molecular mechanisms

摘要

Objective: To explore the protective effect of calcium dobesilate combined with benazepriltherapy on renal injury in patients with early diabetic nephropathy and the possible molecularmechanisms. Methods: A total of 50 patients with early diabetic nephropathy treated in ourhospital between May 2012 and January 2016 were collected, and according to the randomnumber table, the patients were divided into observation group (n=25) and control group (n=25).On the basis of conventional treatment, control group of patients received benazepril therapy,observation group of patients received calcium dobesilate combined with benazepril therapy,and the treatment lasted for 3 months. Before and after treatment, automatic biochemicalanalyzer was used to detect the levels of renal injury indexes in peripheral blood, RIA methodwas used to detect the levels of renal injury indexes in urine, ELISA method was used todetect the levels of renal fibrosis indexes and Western-blot method was used to detect theprotein expression of TGF-β1/BMP-7 and Smad signaling pathway molecules in renal tissue.Results: Before treatment, differences in renal injury index levels, renal fibrosis index levelsand signaling pathway molecule protein expression were not statistically significant betweentwo groups of patients. After treatment, BUN, SCr and β-TP levels in the peripheral blood aswell as KIM-1 level in urine of observation group were lower than those of control group; renalfibrosis indexes TGF-β1, CTGF, TIMP-1, LN and HA levels in serum of observation groupwere lower than those of control group; TGF-β1 and Smad2/3 protein expression in renaltissue of observation group were lower than those of control group while Smad7 and BMP-7protein expression were higher than those of control group. Conclusion: Calcium dobesilatecombined with benazepril therapy can reduce the renal injury and inhibit the fibrosis processin patients with early diabetic nephropathy, and it achieves the above effect by regulating theTGF-β1/BMP-7 and Smad signaling pathway function.