Effect of hTERC and FOXP3 gene magnification in condyloma acuminate lesion on the immune response and cell apoptosis

摘要

Objective: To study the effect of hTERC and FOXP3 gene amplification in condylomaacuminate lesions on the immune response and cell apoptosis. Methods: The condylomaacuminate lesions diagnosed between May 2014 and October 2016 and the normal skin tissuefrom circumcision during the same period were collected to extract RNA and then determinehTERC, FOXP3 and apoptosis-related gene mRNA amplification, and after protein extraction,the protein levels of immune response-related cytokines were determined. Results: hTERC,FOXP3, Livin and Survivin gene mRNA amplification as well as IL-4 and IL-10 protein levelsin condyloma acuminatum lesions were significantly higher than those in normal skin tissuewhile TRAIL, Caspase-3 and PDCD4 mRNA amplification as well as IL-2 and TNF-α proteinlevels were significantly lower than those in normal skin tissue; IL-2 and TNF-α protein levelsin condyloma acuminatum lesions with high FOXP3 mRNA expression were significantlylower than those in condyloma acuminatum lesions with low FOXP3 mRNA expression whileIL-4 and IL-10 protein levels were significantly higher than those in condyloma acuminatumlesions with low FOXP3 mRNA expression; Livin and Survivin mRNA amplification incondyloma acuminatum lesions with high hTERC mRNA expression were significantly higherthan those in condyloma acuminatum lesions with low hTERC mRNA expression whileTRAIL, Caspase-3 and PDCD4 mRNA amplification were significantly lower than those incondyloma acuminatum lesions with low hTERC mRNA expression. Conclusion: Highlyexpressed hTERC and FOXP3 genes in condyloma acuminatum lesions can inhibit apoptosisand inhibit antiviral immune response respectively.