Attenuated Mycobacterium Farcinogenes Strain A24 As A Protective Vaccine Candidate Against Bovine Farcy

摘要

The present study was designed to develop a live vaccine against bovine farcy. A wild strain of Mycobacterium farcinogenes was subjected firstly to serial passage (20) in modified Sauton`s broth then in guinea pigs. The result of the vaccination in calves revealed that 75% of calves in the vaccinated group were protected and withstanded the challenge infection with a virulent freshly isolated strain M. farcinogenes. In addition to pathological and clinical assessment, ELISA was used to monitor the humoral antibody response where the highest antibody titer was achieved at the 5th week in calves post-vaccination. We came to conclusion that the attenuated M. farcinogenes A24 could be used to induce a protective immunity against bovine farcy, and further evaluation of the vaccine should be carried under field condition.