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Supporting data for the characterization of PNA–DNA four-way junctions

机译:表征PNA-DNA四向连接的辅助数据

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Holliday or DNA four-way junctions (4WJs) are cruciform/bent structures composed of four DNA duplexes. 4WJs are key intermediates in homologous genetic recombination and double-strand break repair. To investigate 4WJs in vitro , junctions are assembled using four asymmetric DNA strands. The presence of four asymmetric strands about the junction branch point eliminates branch migration, and effectively immobilizes the resulting 4WJ. The purpose of these experiments is to show that immobile 4WJs composed of DNA and peptide nucleic acids (PNAs) can be distinguished from contaminating labile nucleic acid structures. These data compare the electrophoretic mobility of hybrid PNA–DNA junctions vs . i) a classic immobile DNA 4WJ, J1 and ii) contaminating nucleic acid structures.
机译:霍利迪或DNA四向连接(4WJ)是由四个DNA双链体组成的十字形/弯曲结构。 4WJ是同源基因重组和双链断裂修复的关键中间体。为了在体外研究4WJ,使用四根不对称DNA链组装了连接点。围绕连接分支点的四个不对称链的存在消除了分支迁移,并有效地固定了所得的4WJ。这些实验的目的是表明可以将由DNA和肽核酸(PNA)组成的固定4WJ与污染不稳定的核酸结构区分开。这些数据比较了杂化PNA-DNA连接与的电泳迁移率。 i)经典的固定DNA 4WJ,J1和ii)污染核酸结构。

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